I am so sorry I haven't posted since Sunday! This week has been crazy! Monday I didn't have any WiFi connection at all. Not even during work, so that made my day fun... Not... Yesterday we went to Zwai to gather the forages for planting in Debre Zeit and didn't get home until really late at night. Today was spent planting in Debre Zeit! Busy busy!
Monday:
We started our week off with our usual Monday Morning Meeting, and it went a lot quicker because we had a lot of people missing. Ricardo, Edwin, and a few others were spending the day in Zwai and Debre Zeit so Ricardo could have everything ready for planting on Wednesday. Esther was sick, and a few other workers were absent because they decided to take leave that day. The day still went on and I think everyone got done what they needed!
When Millie and I stopped in Dr. Jean's office that morning, she told me I have a new flower that will be a part of my project! Woo! Someone traveled to the field in Soto, another one of ILRI's fields, last week to look at the field and crops growing there, and took samples of another flower there that flowers, but doesn't produce seed. It's another
Canavalia sp., but no one knows the species of this one either, but know it's not the same species as the one I was already studying. Super! The samples were placed in the fridge right away last week, so Dr. Jean said there was a possibility that the pollen hasn't dried out yet. Before it does dry out, I need to test it right away to see if it is viable and will germinate. My morning was busy! Right after our Monday Morning Meeting ended, I hurried back to the herbarium and set to work! First, I had to figure out if there was even any pollen there, so I spent the next couple hours examining and dissecting a handful of the samples and recording my observations. I found 2 matured flowers that contained pollen, so I set those aside for germination later. For the other flowers and buds that didn't contain pollen, I just recorded my observations of them.
Some background knowledge of my new
Canavalia sp.: Its accession number is 12761, so it’s not the same species as my other
Canavalia that I’m studying because the
accession number for that species is 1251. Also, you can tell right away it’s
not the same species because the flowers are a completely different purple
darker purple, whereas 1251’s petal color is lavender.
Canavalia sp.
is also grown in the greenhouses, but that plant hasn’t ever even produced any
flowers. There are for sure two factors contributing to this. The first one is
the environment. Canavalia sp.
prefers to grow and produce in hot, steamy weather conditions. This is not the
typical weather in Ethiopia, especially not during the rainy season. The other
factor contributing is that it is growing in a pot. This doesn’t give the roots
the desired amount of space to grow. It needs roots to grow very deep into the
soil, and the pot it is growing in isn’t very deep.
On June 23, 2014, I took some cuttings of it and placed
them into glass jars. I only used two jars, but put many cuttings in each. The
process to take cuttings is fairly simple. First, I filled the glass jars with
water about three-quarters of the way full. Then I located some good-sized
stems where I could take my cuttings from. You have to take a certain amount of
the stems in order for it to root. I took a section of the stem that contained
two sections of leaves, and then I made my cut. I tried to go as close to the
bottom of the plant to make my cut. That way I could have many cuttings per
stem. It was slightly difficult because Canavalia
sp.is a vine, so all of the stems were intertwined with one another. It
took about 30 minutes to collect all of the cuttings desired. I placed them all
in the glass jars full of water. Then I left them sitting in the greenhouse to
root. All I need to do is make sure that the jars stay full of water. This is
what helps the roots grow and the stems to stay alive. The time it takes for it
to root can take anywhere from one week to one month or so. Canavalia sp. is a slow plant to root,
so it will probably be closer to a month until is roots. After it roots, each
one will then be planted into a small pot to start growing, and the more and
more it grows, the larger the pot it will be planted in. After they grow to a
decent size, Dr. Jean said that she wants to plant some of them in Zwai to see
how they grow there.
I recorded some observations while examining the flowers
under the microscope. The main purpose of observing them under the microscope
was to see if there was any pollen. That is the first thing to look at if a
plant isn’t seeding. I only observed four flowers this time. Mostly all of the
flowers that were picked as samples were fairly matured. There were only four
flowers that either didn’t have any petals showing, or they were just rising
above the sepals. I looked at three fairly matured flowers and one that was
just beginning to mature. None of the petals were completely opened up yet to
reveal the stamen and carpel, so I had to remove all the petals while
dissecting in order to observe them. The three fairly matured flowers weren’t
completely opened up, but it didn’t take a lot of effort to open them. It
wouldn’t have been much longer until they opened up. The smaller one I
dissected just barely had its petals rising above the sepals. The petals were
above the sepals by 0.4 centimeters. There were completely closed, so it took a
little work to remove the sepals and petals so I was able to observe the
anthers. One the three matured flowers, all ten of the anthers contained
pollen, as well as a small amount of pollen on the stigma and traces of it
going down the outside of the style. On the flower just beginning to mature,
there was only one anther that contained pollen. The other anthers were a pale
yellow. Other than that one anther, there was no sign of pollen.
It was time to germinate! As I said before, pollen dries out within 24 hours, but since the flowers
were placed in the refrigerator as soon as possible, there was a chance the
pollen would still work. I used the sucrose solution that was made on June 10,
2014 to germinate the pollen. I placed one drop of the sucrose solution on the
right side of the slide, then used the forceps to knock the pollen off the
anthers and into the solution. I repeated this on the left side of the slide. I
then used a different flower containing pollen and repeated this process. This
way I would have more data to look at. I didn't check to see if the pollen had
germinated yet because I used the staining solution after that so that the
germinated pollen would stain. This will help me see the germinated pollen
easier as well as see if the pollen is viable or not. There is a chance it
might not show anything because it was over 24 hours since it was picked.
I don't remember if I said anything about the staining process, so here goes! The purpose of staining pollen is to see whether or not it's viable. If it is, the staining solution will color it (in this case, a dark blue) and the rest of the pollen will be a much lighter color. Since we already had the solution made last week, the process of actually staining pollen is a breeze! (Well... kind of... never mind... not so much...) Since I already germinated the pollen and had it sit on the incubator for the 4 hours, it was time to stain! On top of one side of the sucrose, I put 2 drops of stain. Then, I had to hold the slide over a flame until the solution came to a boil. Next, I have to have it sit for 10-15 minutes to stain the pollen and cool off. Lastly, I put a cover slip on top and I'm ready to look at it under the microscope! Observing it, I didn't see any germinated pollen, but Dr. Jean said that she'll have a look at it with me on Thursday after all our field work is done.
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Safety first! Lab coat? Check!
Gloves? Check! |
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Getting some stain in the pipette |
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Dropping staining solution on
the slides |
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Lighting the match for the flame to boil my samples |
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Boiling my sample |
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Boiling my sample |
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Our dinner! |
That was my Monday for the most part! Besides the fact that I didn't post anything about my day because I didn't have any WiFi connection!
Tuesday:
Today started bright and early! 5:30 in the morning to be exact! We were traveling to Zwai today to collect all of Ricardo's plants for his thesis project, and it's a 3 hour drive to get there.
We needed an early start to beat traffic and get a lot done, so that's why we left at 5:30! The sites I saw were amazing on our way there and back! It's a whole different look than Addis. I really loved it! We also dropped in altitude by about 500 meters or so, so that was good for a change!
When we finally got to the field in Zwai, it was a little after 8:30. We got shown around the field for a little bit, I was told where my Canavalia sp. is located so I could gather some samples to test the pollen, and then were instructed to go wander around for a little while! I went to gather my Canavalia sp. samples right away so that I wouldn't be rushed later in the day to collect them! The plant looked way different that I imagined! I didn't know it was planted in one of the plots! I thought it was being grown off to the side of the field!
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The flower |
After gathering my samples, Millie, Andreas, and I decided to listen to directions and wander around for a while since none of us have ever been here before! We were told there was a lake nearby and to look at it, so we headed out in that direction! Right away, Millie and I were mesmerized by the view! So of course we snapped some pictures!
When we were both done drooling at the mountains, (not literally drooling I promise) it was time to go exploring! Millie was fascinated with a bird she saw and wanted to take a picture of it, but it flew off into some woods. She followed it, so we all followed her. We then saw a bridge and road on the other side of the fence and wanted to go there, but didn't know where that road started, so we found an opening in the fence, and I was the first to climb through! The "opening" was more of a small hole about a foot in diameter. Since I am the smallest of the 3 of us and the most daring, I went first to go through. It wasn't the most fun. While going through, I got stabbed many times by thorns, sticks, and who knows what else! But I made it! Two men were riding a motorcycle and were watching me the entire time I was doing this, and as soon as I made it onto the road they said, "You are very strange, but in a special way!" I'm not really sure how to take that, so I took it as a compliment! Millie and Andreas eventually made it through and onto the road as well, so it was time for exploring! The road is highly populated with cabs, people (mainly children) herding goats, mules, and cattle, walkers, and motorcycles. There was never a calm moment!
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About a 6-year-old herding cattle |
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Motorcycle! |
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Cab! It's so tiny! |
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Herd of cattle |
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Herd of cattle and mule |
After some time of exploring and wandering, we figured we should get back and do some actual back-breaking work! When we returned, we were told to sort out the bags that each accession will go into after it's dug up. Ricardo is planting 145 different accessions, so that was a lot of bags to sort and put in number order! Wait... That wasn't back-breaking labor... Here it comes! When the bags were all ordered, other workers then took them to the correct plot number where the accessions were going to be dug up and put in the bags. Our job was to then carry all the bags from the field to near the car where we would then sort them again! Some bags were pretty light, while others weighed a ton! 145 bags later... we had them all near the car and it was time to organize them so that planting them tomorrow will go smoothly!We put the bags in rows of 20 because that was all we could bundle together.
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Look at those straight rows:) |
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So many bags! |
Finally we were done bundling, and it was time to load up the truck! We probably should have thought how much space those bags were going to take because that was a struggle trying to squish all the bags in the bed of the truck... Just loading them all up took a good few hours! It took about 20 men to load it up! We got all the bags loaded by a little after 4 pm, and it was time to head to Debre Zeit to drop them off! We went a little way, and we all decided it would be extremely late when we got home if we drove to Debre Zeit, unloaded all the bags, put them in the shed, then headed home again. Instead, we just drove straight home and would unload the bags in the morning right before we started planting them! We got home so late! It was almost 8 pm when we arrived home, so Millie and I both said goodnight to each other and headed to our rooms! I was so exhausted from our day, there was no way I could write up a blog! Also, my internet was still acting kind of funky still, so I didn't feel like staying up forever just trying to write a blog. I made a quick supper, got ready for bed, and fell asleep instantly! Tomorrow would be even more exhausting I knew, so I needed all the energy I could get!
Today (Wednesday):
Woohoo!!! Another 5:30 departure morning! I didn't want to get out of bed this morning... I didn't get my usual amount of sleep last night, and I was still worn out from yesterday, so I just laid in my bed for a little while... We left right at 5:30 this morning again! (All of us have been so punctual!) It's only an hour and a half drive to Debre Zeit, so that wasn't too bad. We got there around 7, and were supposed to start planting, but it was pouring outside, and we didn't want to work in the rain and dig mud holes, so we decided to wait it out a bit. We finally headed out into the field a little before 8 even though it was still raining. It wasn't raining too hard, so we decided to tough it out. We also didn't want to be there all day planting! In a little while, the rain stopped, so things started to dry up! Millie, Andreas, and I had our first job to line up all the bags in their proper spot to get planted. That took a while because there's 145 accessions (or different bags with plants in them) to line up, and each row is over a foot apart! Talk about a hike just to place a bag in its spot! Finally we were done with that, but by then we were soaked from the rain and wet bags AND muddy from the bags sitting in the mud! Woo! It wasn't even 9 in the morning and I already look like I've done hard work!
Anyway! So after we were done placing the bags, we started to help plant! That was super muddy! My hands were covered in mud for the rest of the day from that! I only planted for a half hour or so, then switched jobs. I don't know if it was because I wasn't doing a good enough job or they just didn't want me to help plant, but my next job was to take a bag, have a woman take handfuls of the plant inside of it, place it in a hole for others to plant, lead her to the 2nd block, take handfuls of the plant inside of it, place it in a hole for others to plant, lead her to the 3rd block, take handfuls of the plant inside of it, place it in a hole for others to plant, make sure there aren't any plants left inside the bag, and start the whole process over with a new bag! If this confused you, let me explain. Block 1 is where we originally lined up all the bags. After planting each accession in block 1, we then carry the bag to its next assigned spot in block 2. After it's planted there, we carry the bag to its assigned spot in block 3. It could be #41 in block one, #242 in block 2, and #417 in block 3. Ricardo made the plan of the field and where each accession is to be planted, so I just followed the numbers on the bag and carried it to the correct spot! We finished the entire field before lunchtime! It was probably record timing! We were all starved, muddy, and ready to wash up!
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The field before we started |
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Beautiful! |
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I love the view! |
Weird story: There was a mistake with some of the accessions. Some of them were planted in the wrong rows, thus causing problems. We had to fix that, so a few of us stayed back to help Ricardo. Millie, Andreas, and about 3 other women stayed to help. While Ricardo was walking up and down the rows figuring out where the problem was, the women started talking to each other and pointing at Millie and me. We didn't know what they were saying, but they later got our attention and pointed the their skirts. We were like, "Ohhhhh no I don't have any skirts. I wear jeans." They just started laughing more, and we didn't know why... Ricardo found the problem and told us where to plant again, so we started doing that and they started talking and laughing again. Then, one of the women said something to Andreas in Amharic, and he translated it to us. They said they felt bad for us. I asked them, "Why?" He then translated that to them and they responded again saying because Millie and I are all muddy. Umm... yeah of course I'm muddy... I was planting, carrying bags, and a lot of other stuff in a field when it was raining. I don't understand why they felt bad... It's just mud... Who knows! Later, when we were done planting, one of them said a comment about Millie and my watches. We showed our to them and they laughed again. One of them said their friend (who was standing right next to her) wanted it as a gift, so Millie said that was okay and started taking hers off. They freaked out and said they didn't want it. We were both so confused... They just asked for it, but when offered, they freaked out and said they didn't anymore. I have no idea. It was really strange but interesting. Great day!
Now I'm all caught up on my blogs, and am off to bed! We're planting in Addis tomorrow and I have to be to work around 8 in the morning to help plant! I can't wait! Time to get all muddy again! Woo! I love the field work though:) It's great to be out of the office and lab once in a while and see how planting here goes. Well goodnight and I hope this wasn't too painful to read! Sorry!